Human IgE (non-immune), biotinylated
Human monoclonal antibody
Cat.No. DIA HE1B
Preparation: Protein L purified, Biotinylated
Content: Available in 100 µL, 1mg/mL
Solvent:0.01 M phosphate buffer, pH 7.4, with 0.15 M NaCl and 0.09 % azide
Storage: Store at 2 to 8ºC. Do not freeze
This product is purified human IgE with kappa light chains produced in vitro from a monoclonal hybridoma. Original material is obtained from a healthy donor tested negative by US-FDA approved tests against HIV, HCV and Hepatitis B.
Must be handled as potentially infectious as all human material.
The purity of the human IgE is > 98%, tested by SDS-PAGE.
As the IgE comes from a monoclonal cell line, there is no contamination of antibodies of other isotypes. The human IgE is purified by Protein L chromatography. The remaining contaminants are mainly components from Foetal Bovine Serum.
Non-immune.
This antibody is well suited as a standard in IgE quantifying assays due to its very low batch-to-batch variation. Other applications include immunochemistry and cellular immunology research.
Larger Picture (113kB)
Picture: Lane 1 shows molecular weight markers, lane 2 Diatec purified human IgE, batch 307, MW 185 000, lane 3 shows myeloma source IgE, purified from human serum.
Downloads
DIA HE1B.pdf (32.79 kB) Product specification
MSDS ABS Biotin-Mabs.pdf (52.95 kB)
Flyer IgE.pdf (302.37 kB)
IgE flyer
